product information background SVR4-like (Supressor of variegation 4-like) is a homolog of SVR4. It is a nuclear-encoded chloroplast-located protein required for proper function of the plastid transcriptional machinery. Synonymes: MRL7-like, MESOPHYLL-CELL RNAI LIBRARY LINE 7-LIKE, MRL7-L. immunogen KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SVR4-like protein sequence, UniProt: Q84RF5, TAIR: At2g31840 antibody format rabbit polyclonal serum lyophilized quantity 50 µl for reconstitution add 50 µl, of sterile water. storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. tested applications western blot (WB) related products AS13 2725 | SVR4 | Supressor of variegation 4, rabbit antibodies additional information This protein is present in very low amounts only in early stages of plant development and this has to be taken into account when harvesting the tissue. Western blots were done on: Arabidopsis thaliana total protein extract from cotyledons and Hordeum vulgare (2-14 days old plants).

application information
recommended dilution		1 : 1000 with standard ECL (WB)
expected | apparent MW		40 | 42 kDa
confirmed reactivity		Arabidopsis thaliana, Horderum vulgare
predicted reactivity		please, inquire
not reactive in		no confirmed exceptions from predicted reactivity are currently known
additional information
selected references		Powikrowska et al. (2013). SVR4(suppressorofvariegation4) andSVR4-like:twoproteinswith aroleinproperorganizationof thechloroplastgeneticmachinery. Physiol Plant. Sep 23. doi: 10.1111/ppl.12108.

application example

SVR4-like antibody tested on intact chloroplasts isolated from ome week old barley. Intact chloroplasts were isolated from 1 week old Hordeum vulgare plants. The sample corresponding to 4ug Chl and was separated on 12% Criterion XT Bis-Tris SDS-PAGE (BioRad) gels and blotted for 25min 100V to PVDF membrane. Blot was blocked with 5% fat free skimmed milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 overnight with agitation in 4˚C. The antibody solution was decanted and the blot was washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (swine anti-rabbit IgG horse radish peroxidase conjugated, from Dako) diluted to 1: 5000 in 1% fat free skimmed milk in PBS-T for 1h at RT with agitation. The blot was washed 5 min in PBS-T and 1 min in PBS developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 20 min.

Courtesy of Dr. Małgorzata Powikrowska, University of Copenhagen, Danmark