Description:

Rabbit polyclonal antibody

  Background:

PSBG is a protein which was originally thought to be part of photosystem II. 

  Synonyms:

PSBG, NDHK, PHOTOSYSTEM II REACTION CENTER PROTEIN G

  Immunogen:

KLH-conjugated synthetic peptide (17 aa from N terminal section) derived from Arabidopsis thaliana PSBG (ATCG00430).

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

25 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Zea mays, Oryza sativa, Medicago truncatula, Solanum tuberosum, Nicotiana tabacum, Solanum lycopersicum, Vitis vinifera, Physcomitrium patens, Triticum aestivum, Hordeum vulgare, Medicago truncatula,Panicum virgatum, Setaria viridis, Leymus chinensis, Sorghum bicolor, Cucumis sativus, Populus trichocarpa, Gossypium raimondii, Brassica napus, Glycine max, and 80-99% homologues with the sequence in Spinacia oleracea.
For more species homologues information, please contact tech support at tech@phytoab.com.

1 is 4 μg thylakoid membrane protein from Arabidopsis thaliana.
2 is 4 μg thylakoid membrane protein from NDH mutant of Arabidopsis thaliana.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.