Description:

Rabbit polyclonal antibody

  Background:

PsaF encodes subunit F of photosystem I which participates in efficiency of electron transfer from plastocyanin to P700 (or cytochrome c553 in algae and cyanobacteria). 

  Synonyms:

PsaF, PHOTOSYSTEM I SUBUNIT F, PSAF

  Immunogen:

KLH-conjugated synthetic peptide (18 aa from C terminal section) derived from Arabidopsis thaliana PsaF (AT1G31330).

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube". 

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

24 / 17 kDa 

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Medicago truncatula, Brassica rapa, Brassica napus, and 80-99% homologues with the sequence inSpinacia oleracea, Oryza sativa, Hordeum vulgare, Panicum virgatum, Gossypium raimondii, Zea mays, Triticum aestivum, Setaria viridis, Solanum tuberosum, Nicotiana tabacum, Solanum lycopersicum, Populus trichocarpa, Medicago truncatula, Glycine max.
For more species homologues information, please contact tech support at tech@phytoab.com.

1 is 0.5 μg thylakoid membrane protein from Arabidopsis thaliana.
2 is 0.5 μg thylakoid membrane protein from PSI mutant of Arabidopsis thaliana.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.