Description:

Rabbit polyclonal antibody

  Background:

In chloroplasts, transcription of plastid genes is mediated by two types of RNA polymerase: plastid-encoded RNA polymerase (PEP) and nuclearencoded RNA polymerase (NEP). Transcription in plastids is also mediated by a number of nuclear-encoded factors in addition to PEP and NEP. In the insoluble RNA polymerase preparation samples, a total of 18 components named as pTACs (pTAC1 to pTAC18) were identified. pTAC3 (AT3G04260) is one of the components associated with PEP complex.

  Synonyms:

pTAC3, PDE324, PIGMENT DEFECTIVE 324, PLASTID TRANSCRIPTIONALLY ACTIVE 3, PTAC3

  Immunogen:

KLH-conjugated synthetic peptide derived from central section of pTAC3 protein in Arabidopsis thaliana AT3G04260.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

103 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana, Zea mays, Oryza sativa

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Oryza sativa Japonica Group, Gossypium raimondii, Spinacia oleracea, Populus trichocarpa, Zea mays,Glycine max, Setaria viridis, Sorghum bicolor, Hordeum vulgare, and 80-99% homologues with the sequence inBrassica rapa, Brassica napus, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera, Nicotiana tabacum,Cucumis sativus, Medicago truncatula.
For more species homologues information, please contact tech support at tech@phytoab.com.

Lane 1: 5 μl total chloroplast protein from Arabidopsis thaliana leaf.
Lane 2: 5 μl total chloroplast protein from Zea mays leaf.
Lane 3: 5 μl total chloroplast protein from Oryza sativa leaf.
Electrophoresis: 10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.