Description:

Rabbit polyclonal antibody

  Background:

Rps7 is one of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit.

  Synonyms:

RPS7, RIBOSOMAL PROTEIN S7

  Immunogen:

KLH-conjugated synthetic peptide of RPS7 derived from Arabidopsis thaliana ATCG00900 ATCG01240.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:4000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

17 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Medicago truncatula, Glycine max, Gossypium raimondii, Brassica rapa, Nicotiana tabacum, Triticum aestivum, Sorghum bicolor, Zea mays, Vitis vinifera, Solanum tuberosum, Spinacia oleracea, Hordeum vulgare subsp. Spontaneum, Oryza sativa, and 80-99% homologues with the sequence in Brassica rapa, Populus trichocarpa, Cucumis sativus, Brassica napus.
For more species homologues information, please contact tech support at tech@phytoab.com.

10 μl total chloroplast protein from Arabidopsis thaliana.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:4000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.