clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
PRODUCT INFORMATION
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background |
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TPS1 (Trehalose-6-phosphate synthase 1), EC=2.4.1.15 is an enzyme required for normal embryo development, vegetative growth and transition to flowering. It is involved in the regulation of glucose sensing. Expressed in seedlings, leaves, roots, stems, flowers and siliques. Up-regulated during seed development. Alternative names: alpha-trehalose-phosphate synthase [UDP-forming] 1. |
immunogen |
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KLH-conjugated synthetic peptide derived from Arabidopsis thaliana TPS1, UniProt: Q9SYM4, TAIR:AT1G78580 |
antibody format |
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rabbit |
polyclonal |
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serum |
lyophilized | |
quantity |
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50 µl |
for reconstitution add 50 µl, of sterile water. | |
storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
tested applications |
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western blot (WB) |
related products |
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AS05 086 | UGPase | UDP-glucose pyrophosphorylase (cytoplasm marker), rabbit antibody
collection of antibodies involved in carbohydrate metabolism |
additional information |
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to be added when available | |
APPLICATION INFORMATION
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recommended dilution |
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1 : 5000 with standard ECL (WB) |
expected | apparent MW |
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105.9 kDa |
confirmed reactivity |
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Arabidopsis thaliana
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predicted reactivity |
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Camelia sinensis |
not reactive in |
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no confirmed exceptions from predicted reactivity are currently known |
additional information |
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so far this antibody was not used on endogenous extracts from Arabidopsis thaliana |
selected references |
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to be added when available, antibody released in March 2014. |
application example
10 µg of total protein from Nicotiana benthamiana leaves expressing AtTPS1-GFP were separated on 10% SDS-PAGE using semi-dry transfer and blotted 1h to PVDF. Crude: non-purified material. Purified: immunoprecipitated with anti-GFP beads. Blots were blocked with 5% skimmed milk powder dissolved in TBS-T (0.1 % Tween 20) at 4°C ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 for 1h at room temperature (RT) with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 25 000 for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Immobilon Western Chemiluminescent HRP reagent according to the manufacturer's instructions. Exposure time was 30 seconds.
Courtesy of Dr. Takeo Sato, Hokkaido University, Japan
bio-equip.com
北京啟維益成科技有限公司（Beijing QiWei YiCheng Tech.,Ltd.(Qwbio)）成立于2005年12月，由從事生物領(lǐng)域多年銷售經(jīng)驗(yàn)人士創(chuàng)立。目前我公司已經(jīng)與幾十家著名的試劑供應(yīng)商建立了長期穩(wěn)定的合作關(guān)系，已成為眾多國際知名品牌的中國總代理或一級代理商；并且為滿足國內(nèi)客戶的最新需求，我們也與時俱進(jìn)地不斷與世界上更多的知名品牌洽談合作代理事宜；企業(yè)的主要目標(biāo)是把國外有特點(diǎn)的生物前沿產(chǎn)品引進(jìn)中國，讓國內(nèi)科學(xué)研究機(jī)構(gòu)和制藥企業(yè)盡早使用上最新型的科研產(chǎn)品。
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