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                                                              MME4 | malic enzyme
                                                              英文名稱:MME4 | malic enzyme總訪問(wèn):592
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                                                              產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                                              規(guī)       格:AS122617 最后更新:2024-12-5
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                                                              product information
                                                              background

                                                              MME4 (malic enzyme) belongs to the malic enzyme family.

                                                              immunogen

                                                              KLH-conjugated synthetic peptide derived Chlamydomonas reinhardtii MME4 protein seqeunce A8IUZ4. The peptide is also conserved in MME3 A8IUW2

                                                              antibody format

                                                              rabbit

                                                              polyclonal

                                                              affinity purified serum, in PBS pH 7.4

                                                              lyophilized

                                                              quantity

                                                              200 µg

                                                              for reconstitution add 200 µl, of sterile water.

                                                              storage

                                                              store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                                              tested applications

                                                              western blot (WB)

                                                              related products AS12 2618 | anti-FMR | fumarate reductase
                                                              additional information

                                                              to be added when available

                                                              application information
                                                              recommended dilution

                                                              1 : 1000 with standard ECL (WB)

                                                              expected | apparent MW

                                                              56 kDa

                                                              confirmed reactivity

                                                              Chlamydomonas reinhardtii

                                                              predicted reactivity Chlamydomonas reinhardtii
                                                              not reactive in

                                                              no confirmed exceptions from predicted reactivity are currently known

                                                              additional information

                                                              to be added when available

                                                              selected references

                                                              to be added when available, antibody released in November 2012.


                                                              application example

                                                              western blot using anti-MME4 antibody

                                                              25 µg of total protein from Chlamydomonas reinhardtii, oxic conditions and dark anoxia were separated on 4-15 % SDS-PAGE and blotted 1h to PVDF. Blotting was done using SNAP-ID kit: incubation in blocking buffer for 1 min., following incubation in a primary antibody at a dilution of 1: 1 000 for 20 min, wash three times with wash buffer TBS-T, followed by incubation in a secondary antibody at a dilution of 1: 5000, for 20 min. and three times wash in TBS-T. The blot was washed and developed with AP color development reagent (AP conjugate substrate kit from Bio-rad) according to the manufacturer's instructions.

                                                              Courtesy of Dr. Alexandra Dubini



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