product information background AP2 (Floral homeotic protein APETALA 2) is a transcription factor involved in the specification of floral organ identity, establishment of floral meristem identity and controlling seed mass. immunogen KLH-conjugated synthetic peptide derived from AP2 sequence of Arabidopsis thaliana, UniProt: P47927, TAIR: At4g36920 antibody format rabbit polyclonal affinity purified serum lyophilized quantity 50 µg for reconstitution add 50 µl of sterile water. storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. tested applications western blot (WB) related products AS11 1773 | WUSCHEL-related homeobox 4, rabbit antibody AS11 1759| WUS | WUSCHEL protein, rabbit antibody additional information Affinity purified antibodies are lyophilized from PBS pH 7.4 with 0.02 % sodium azide

application information
recommended dilution		1 : 6000 with standard ECL (WB)
expected | apparent MW		47.8 kDa
confirmed reactivity		Arabidopsis thaliana, Brassica napus, Oryza sativa
predicted reactivity
not reactive in		no confirmed exceptions from predicted reactivity are currently known
additional information
selected references		to be added when available, antibody released in December 2013

application example


1 µg of total protein from Oryza sativa lines grown under drought stress conditions and over expressing protein DREB (AP2 domain, 285 aa, 31.35 kDa) (lines 1-5) and purified 100 ng of DREB (line 6), extracted with were separated on 12.5 % SDS-PAGE and blotted 4h to Nitrocellulose membrane. Blots were blocked with blocking agent 5% BSA or 1X TBS, 0.1% Tween-20 with 5% w/v non-fat dry milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 6 000 for 1h at RT or overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 x 5 min in 1XTBST at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:8000 in for 1h at RT or max. 2h with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was preferred between 20 to 30 seconds or max. 1 min.

Courtesy of Dr. Sunita Yadav, Indian Institute of Technology Kharagpur, India