product information background PR-2 (Pathogenesis-related protein 2) is involved in the defence of plants against pathogens. This protein has a catalytic activity and is hydrolysing of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans, EC=3.2.1.39. Alternative names:Glucan endo-1,3-beta-glucosidase, acidic isoform,(1->3)-beta-glucan endohydrolase, Beta-1,3-endoglucanase, Beta-1,3-glucanase 2 immunogen KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PR-2 UniProt P33157, TAIR AT3G57260 antibody format rabbit polyclonal serum lyophilized quantity 50 µl for reconstitution add 50 µl, of sterile water. storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. tested applications western blot (WB) related products AS10 687 | PR-1 | Pathogenesis-related protein 1, rabbit antibodies AS07 208 | PR-2 | GLU I | class I beta-1,3-glucanase, rabbit antibodies for other species, not Arabidopsis thaliana AS07 207 | PR-3 | CHN | class I chitinase, rabbit antibodies AS12 2369 | PR-4 | Pathogenesis-related protein 4, rabbit antibodies AS12 2373 | PR-5 | Pathogenesis-related protein 5, rabbit antibodies collection of antibodies to other proteins involved in a response to pathogen attack additional information

application information
recommended dilution		1 : 1000 with standard ECL (WB)
expected | apparent MW		37.3 kDa (processing aa 1-30, mature peptide 34.1 kD)
confirmed reactivity		Arabidopsis thaliana
predicted reactivity		Brassica juncea, Brassica oleracea, Citrus chinensis, Glycine max, Litchi chinensis, Manihot esculenta, Nicotiana tabacum
not reactive in		no confirmed exceptions from predicted reactivity are currently known
additional information		Does not cross-react with other 1,3-beta glucosidases.
selected references		to be added when available, antibody released in August 2013

application example


10 µg of total protein from Arabidopsis thaliana mature leaves were extracted with 8M UREA and were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% Milk in TBS-T 0,05% for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in TBS-T/Milk 5% for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was seconds 60 seconds.

Courtesy of Louis-Valentin Meteignier, PhD student, University of Sherbrooke, Canada