| product information
| | background | | GRF (General regulatory factor 2) or14.3.3’s are 30KDa proteins involved in protein interactions with target proteins containing phosphorylated target sites. Functions of 14.3.3’s include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system). A variety of apparently unrelated biological activities, including a role in development and signal transduction - brassinosteroid mediated signaling pathway. | | immunogen | | conserved surface-exposed peptide conjugated to KLH.This sequence is repeated in all 12 “canonical” members of the Arabidopsis thaliana GRF/14-3-3 family. GRF1 GF14 chi UniProt: F4JJ94, TAIR: AT4G09000,GRF2 GF14 omega, UniProt:Q01525, TAIR:AT1G78300, GRF3 GF14 psi UniProt: F4KBI7 , TAIR:AT5G38480,GRF4 GF14 phi UniProt:F4HWQ5, TAIR: AT1G35160,GRF5 GF14 upsilon UniProt: P42645, TAIR:AT5G16050,GRF6 GF14 lambda UniProt: P48349#P48349-2, TAIR:AT5G10450,GRF7 GF14 nu UniProt:Q96300, TAIR:AT3G02520,GRF8 GF14 kappa UniProt:F4KHY7, TAIR:AT5G65430,GRF9 GF14 mu UniProt: F4IP55, TAIR:AT2G42590, GRF10 GF14 epsilon UniProt: F4I1C1, TAIR:AT1G22300, GRF11 GF14 omicron UniProt: Q9S9Z8, TAIR: AT1G34760, GRF12 GF14 iota UniProt:Q9C5W6 TAIR: AT1G26480. | | antibody format | | rabbit | polyclonal | | serum | lyophilized | | | quantity | | | | storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | tested applications | | western blot (WB) | | related products | | AS08 317 | 14.3.3C | 14.3.3-like protein C, rabbit antibody specific to Hordeum vulgare
matching secondary antibody for chemiluminescence detection
| | additional information | | This antibody is recognizing recombiant GRF of Lilium longiflorum Lil1433_0 accession: AF191746, Lil1433_2, accession: EF397608 and recombinant GRF1,2,3,5 and 6 of Arabidopsis thaliana: GRF1 14-3-3 chi (At4g09000.1),
GRF2 14-3-3 omega (At1g78300.1), GRF3 14-3-3 psi (At5g38480.1),
GRF5 14-3-3 upsilon (At5g16050.1), GRF6 14-3-3 lambda (At5g10450.2),
GRF8 14-3-3 kappa (At5g65430.1), GRF11 14-3-3 omicron (At1g34760.1).
There is also very weak reaction to Physcomitrella patens Pp14-3-3 Pp1s 73_133V6 (closest homolog to AtGRF6) and Chlamydomonas reinhardtii Cr 14-3-3 Cre 12.g559250 (closest homolog to AtGRF6). | |
