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AHK2 | Histidine kinase 2
英文名稱(chēng):AHK2 | Histidine kinase 2總訪問(wèn):426
國(guó)產(chǎn)/進(jìn)口:進(jìn)口半年訪問(wèn):2
產(chǎn)地/品牌:agrisera產(chǎn)品類(lèi)別:植物試劑
規(guī)       格:AS122113 最后更新:2024-12-5
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product information
background

AHK2 (Histidine kinase 2) is a cytokinin receptor, multi-pass membrane protein which transmits the stress signal to a downstream MAPK cascade. Alternative name: Authentic HIS-Kinase 2.

immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AHK2 protein sequence, TAIR: AT5G35750, UniProt: Q9C5U2

antibody format

rabbit

polyclonal

affinity purified serum

lyophilized

quantity

50 µg

for reconstitution add 50 µl of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products AS12 2632 | Anti-AHK4 | Histidine kinase 4, rabbit antibody
additional information

to be added when available

application information
recommended dilution

1 : 1000 with standard ECL (WB)

expected | apparent MW

131 kDa

confirmed reactivity

Arabidopsis thaliana

predicted reactivity Vitis vinifera
not reactive in

no confirmed exceptions from predicted reactivity are currently known

additional information

to be added when available

selected references

to be added when available, antibody released in July 2013.


application information

western blot using anti-AHK2 antibodies


The Arabidopsis thaliana wild-type (WS) and ahk2-2T-DNA insertion mutant plants were grown on MS/2 media for six days.Tissue was collected, frozen in liquid nitrogen, ground in 3 volumes of 2x SDS-PAGE loading buffer and heated at 95 C for 5 min. After 10 min centrifugation at RT, 20 µl of the supernatant was loaded per lane on a 7.5% TGX gel (BioRad). After separation, proteins were blotted 1 hr onto a supported nitrocellulose membrane.Blots were blocked with 10% milk in PBS-T for 15 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody diluted 1: 10, 000 in 1% milk in PBS-T for 16h at 4C with agitation. The antibody solution was decanted and the blot was washed 3 times for1 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 2000 in PBS-Tfor 1h at RT with agitation. The blot was washed as above and developed for 5 min withSuperSignal WestFemtoSubstrateaccording to the manufacturers instructions. Chemiluminescent signal was captured using ChemiDoc equipped with a CCD camera.

Courtesy of Dr. Jasmina Kurepa, University of Kentucky College of Agriculture, Food and Environment, USA

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