| product information
| | background | | DCL3 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Synonymes: Endoribonuclease Dicer homolog 3. | | immunogen | | KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DCL3 sequence, Q9LXW7 , At3g43920 | | antibody format | | rabbit | polyclonal | | affinity purified serum | lyophilized | | | quantity | | 200 µg | for reconstitution add 200µl, of sterile water. | | | storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | tested applications | | western blot (WB) | | related products | | AS12 2102 | anti-DCL1 | Dicer-like protein 1 antibody
| | additional information | | to be added when available | |
application information
|
| recommended dilution | | 1 : 1000 with standard ECL (WB) |
| expected | apparent MW | | 117 kDa |
| confirmed reactivity | | Arabidopsis thaliana
|
| predicted reactivity | | Arabidopsis thaliana |
| not reactive in | | no confirmed exceptions from predicted reactivity are currently known |
| additional information | | Recommend protein load is from 40-50µg/well (using 1.5 mm spacers helps to obtain wider wells) |
| selected references | | to be added when available, antibody released in August 2012. |
application example
50 µg of total proteinfromArabidopsis thalianatotal cell extract which came from the ground, frozen powder and has been directly transferred to 2x Laemmli sample buffer was separated on15 %SDS-PAGEand blotted over night at 64 mA to nitrocellulose (wet transfer). Blots were blocked with Roti-block over night at 4°C agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000(1)and 1: 500(2)1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from AgriseraAS09 602) diluted to 1:10 000 for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 1 min.
Courtesy of Dr. Sascha Laubinger, ZMBP, Germany
bio-equip.com
北京啟維益成科技有限公司（Beijing QiWei YiCheng Tech.,Ltd.(Qwbio)）成立于2005年12月，由從事生物領(lǐng)域多年銷售經(jīng)驗人士創(chuàng)立。目前我公司已經(jīng)與幾十家著名的試劑供應(yīng)商建立了長期穩(wěn)定的合作關(guān)系，已成為眾多國際知名品牌的中國總代理或一級代理商；并且為滿足國內(nèi)客戶的最新需求，我們也與時俱進(jìn)地不斷與世界上更多的知名品牌洽談合作代理事宜；企業(yè)的主要目標(biāo)是把國外有特點(diǎn)的生物前沿產(chǎn)品引進(jìn)中國，讓國內(nèi)科學(xué)研究機(jī)構(gòu)和制藥企業(yè)盡早使用上最新型的科研產(chǎn)品。
我們代理的產(chǎn)品覆蓋細(xì)胞生物學(xué)、植物學(xué)、分子生物學(xué)、免疫學(xué)、生物化學(xué)、蛋白組學(xué)。生物制藥與診斷試劑研發(fā)生產(chǎn)等領(lǐng)域。
細(xì)胞生物學(xué)包含細(xì)胞培養(yǎng)，細(xì)胞遷移，細(xì)胞信號轉(zhuǎn)導(dǎo)等。
植物學(xué)包含植物抗體，植物抗生素，植物組織培養(yǎng)試劑等。
分子生物學(xué)包含表觀遺傳試劑盒，核酸提取，轉(zhuǎn)染試劑等。
免疫學(xué)包含抗體，各類檢測試劑盒等。
生物化學(xué)包含各類生化試劑，化學(xué)小分子，常規(guī)試劑等。
蛋白組學(xué)包含不同種屬的重組蛋白，天然提取蛋白，細(xì)胞因子等。
生物制藥與診斷試劑研發(fā)方面的試劑包含各類蛋白抑制劑，酶類等。
在公司全體員工的共同努力和國外生產(chǎn)商的支持下，在業(yè)務(wù)上實(shí)現(xiàn)了迅猛發(fā)展，相繼在上海、武漢、廣州、長春、石家莊、哈爾濱等重點(diǎn)城市建立了分支機(jī)構(gòu)或授權(quán)服務(wù)商，并以專業(yè)和優(yōu)質(zhì)的服務(wù)成為多家世界500強(qiáng)企業(yè)的中國區(qū)產(chǎn)品供應(yīng)商，以及中國科學(xué)院科研采購平臺的中標(biāo)供貨商。2008年以后業(yè)務(wù)的迅猛發(fā)展促進(jìn)了啟維益成內(nèi)部組織架構(gòu)的不斷優(yōu)化，我們建立了自己的網(wǎng)絡(luò)銷售平臺，業(yè)務(wù)代表銷售平臺，針對科研客戶與企業(yè)客戶的不同需求，綜合網(wǎng)絡(luò)平臺和業(yè)務(wù)代表平臺的優(yōu)點(diǎn)，啟維益成創(chuàng)建了科研用戶與工業(yè)客戶兩支獨(dú)立的營銷團(tuán)隊，分別配備不同的專業(yè)技術(shù)支持和客服人員，通過網(wǎng)絡(luò)和業(yè)務(wù)代表，力求為不同類型的客戶提供前沿專業(yè)的解決方案。
公司客戶遍布中國生命科學(xué)基礎(chǔ)研究、開發(fā)應(yīng)用、制藥、疾病診斷與控制、商品檢驗檢疫、生物技術(shù)公司、食品安全以及農(nóng)林畜牧業(yè)等諸多領(lǐng)域。
我們追求目的是與研究機(jī)構(gòu)及合作伙伴之間誠信而專業(yè)的對話，力求建立長久合作。我們相信我們精心提供的高效、優(yōu)質(zhì)的產(chǎn)品和技術(shù)服務(wù)，會使您實(shí)驗設(shè)計更有效，實(shí)驗操作更簡便，研究結(jié)果更可靠。
放眼未來，公司將努力實(shí)現(xiàn)更大的突破，將國外優(yōu)秀產(chǎn)品不斷引進(jìn)國內(nèi)，同時積極推動國內(nèi)的產(chǎn)品銷往世界各地
