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ABI1 | abscisic acid insensitive 1
英文名稱:ABI1 | abscisic acid insensitive 1總訪問:437
國產(chǎn)/進口:進口半年訪問:5
產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
規(guī)       格:AS121861 最后更新:2024-12-5
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product information
background

ABI1 is a key component and repressor of the abscisic acid (ABA) signaling pathway. It regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane, drought-induced resistance and rhizogenesis, response to glucose, high light stress, seed germination and inhibition of vegetative growth. Expressed in seeds and seedlings. Confined to lateral root caps and columella cells in roots. Induced by low temperature, drought, high salt, ABA and ethylene. Activates/represses SnRK2.6/SRK2E/OST1 in response to ABA-dependent stimuli. Alternative names: ABI1, ABA INSENSITIVE 1, AtABI1, Protein phosphatase 2C 56, AtPP2C56, PP2C56.

immunogen KLH-conjugated peptide, derived from Arabidopsis thaliana ABI1 sequence UniProt: P49597, TAIR: AT4G26080. Chosen peptide is not present in AtABI2.
antibody format

rabbit

polyclonal

affinity purified serum

lyophilized

quantity
50 µg for reconstitution add50 µl of sterile water.
storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.

Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB), immunoprecipitation (IP)

related products AS12 1871 | ABI2 | abscisic acid insensitive 2, rabbit antibody

AS12 1862 | ABI3 | abscisic acid insensitive 3, rabbit antibody

AS12 1872 | ABI4 | abscisic acid insensitive 4, rabbit antibody

AS12 1863 | ABI5 | abscisic acid insensitive 5, rabbit antibody
additional information

It is of crucial importance to chose a material in which ABI1 protein is expressed in detectable amounts, following stress treatment like ozone or drought. This protein is not detectable in plants grown under optimal conditions. This antibody will detect both, recombinant and endogenous ABI1 protein.

application information
recommended dilution 3 µg (WB) , 5 µg (IP for a 200 ul of a cell extract)
expected | apparent MW 47.5 kDa
confirmed reactivity

Arabidopsis thaliana

predicted reactivity please, inquire
not reactive in

no confirmed exceptions from predicted reactivity known in the moment

additional information Important note: blocking with more than 3 % skimmed milk will result in lack of signal for this antibody.
selected references

to be added when available, antibody released in October 2013.


application example


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5 µg of StrepTag-ABI1 protein extracted from Arabidopsis thaliana protoplasts with isolation buffer (20 mM Hepes, pH 7.5, 10 mM MgCl2, 1 mM DTT, 60 mM β-glycerophosphate, 1 mM NaF 1 mM phenylmethylsulfonyl fluoride, 0.1 mM NaVO3, Roche inhibitors)were separated on 12%SDS-PAGEand blotted 1h toPVDF. Blots were blocked with 3% semi-skimmed milk for 30 min. at room temperature (RT) with agitation. Blot was incubated with3 ug ofthe primary antibody for 30 minutes at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602 ) diluted to 1:50 000 in for 30min. at RT with agitation. The blot was washed as above and developed for 1 min with ECL according to the manufacturer's instructions.Exposure time was 5 min.

Courtesy of Dr. Agnieszka Ludwików, Adam Mickiewicz University, Poland
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