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ZCP2 | Zinc Chaperone Protein
英文名稱:ZCP2 | Zinc Chaperone Protein總訪問:321
國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:1
產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
規(guī)       格:AS121848 最后更新:2024-12-5
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product information
background

COG0523 (Zinc defficiency induced protein) is a putative metal chaperone from G3E family of GTPases which acts in metal trafficking.

immunogen

recombinant ZCP2 protein, ID 536252

antibody format

rabbit

polyclonal

serum

lyophilized

quantity

50µl

for reconstitution add 50 µl, of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products collection of antibodies to various Chlamydomonas proteins
additional information
application information
recommended dilution

1 : 1000 with ALP (WB)

expected | apparent MW

70 | 100 kDa (probably due to glycosylation)

confirmed reactivity

Chlamydomonas reinhardtii

predicted reactivity Chlamydomonas reinhardtii
not reactive in

no confirmed exceptions from predicted reactivity are currently known

additional information

This antibody can be used as a marker of zinc deficiency in Chlamydomonas.

selected references

to be added when available, antibody release in May 2012


application example

western blot using anti-COG0523 Zinc deficiency protein

Chlamydomonas reinhardtii soluble proteins (10 µg) were separated on a 7.5% SDS-PAGE gel and blotted to nitrocellulose for 90 min. at 1.5 mA cm-2. The membrane was blocked with 1% milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2 hr at RT with agitation. The antibody solution was
decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in PBS-T + 1% milk at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse alkaline phosphatase conjugated, from Southern Biotech ) diluted to 1:3000 in PBS-T + 1% milk for 45 min at RT with agitation. The membrane was washed 2 times for 5 min in PBS-T + 1% milk at RT with agitation, then rinsed with TBS (pH 7.5), and developed.

Courtesy Dr. Dudley Page, UCLA, USA

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