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                                                              ZCP2 | Zinc Chaperone Protein
                                                              英文名稱:ZCP2 | Zinc Chaperone Protein總訪問:321
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                                                              規(guī)       格:AS121848 最后更新:2024-12-5
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                                                              background

                                                              COG0523 (Zinc defficiency induced protein) is a putative metal chaperone from G3E family of GTPases which acts in metal trafficking.

                                                              immunogen

                                                              recombinant ZCP2 protein, ID 536252

                                                              antibody format

                                                              rabbit

                                                              polyclonal

                                                              serum

                                                              lyophilized

                                                              quantity

                                                              50µl

                                                              for reconstitution add 50 µl, of sterile water.

                                                              storage

                                                              store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                                              tested applications

                                                              western blot (WB)

                                                              related products collection of antibodies to various Chlamydomonas proteins
                                                              additional information
                                                              application information
                                                              recommended dilution

                                                              1 : 1000 with ALP (WB)

                                                              expected | apparent MW

                                                              70 | 100 kDa (probably due to glycosylation)

                                                              confirmed reactivity

                                                              Chlamydomonas reinhardtii

                                                              predicted reactivity Chlamydomonas reinhardtii
                                                              not reactive in

                                                              no confirmed exceptions from predicted reactivity are currently known

                                                              additional information

                                                              This antibody can be used as a marker of zinc deficiency in Chlamydomonas.

                                                              selected references

                                                              to be added when available, antibody release in May 2012


                                                              application example

                                                              western blot using anti-COG0523 Zinc deficiency protein

                                                              Chlamydomonas reinhardtii soluble proteins (10 µg) were separated on a 7.5% SDS-PAGE gel and blotted to nitrocellulose for 90 min. at 1.5 mA cm-2. The membrane was blocked with 1% milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2 hr at RT with agitation. The antibody solution was
                                                              decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in PBS-T + 1% milk at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse alkaline phosphatase conjugated, from Southern Biotech ) diluted to 1:3000 in PBS-T + 1% milk for 45 min at RT with agitation. The membrane was washed 2 times for 5 min in PBS-T + 1% milk at RT with agitation, then rinsed with TBS (pH 7.5), and developed.

                                                              Courtesy Dr. Dudley Page, UCLA, USA

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