| product information
| | background | | Patatin is the most abundant protein in potato (Solanum tuberosum L.) tubers, about 60 % of a total protein. It serves as a storage protein. Mature tuber patatin variants are dimers of 40-42 kDa subunits without disulphide bridges. | | immunogen | | KLH-conjugated synthetic peptide derived from a C-terminal part of 36 known isoforms of patatin from Solanum tuberosum including: Q3YJS9, Q3YJT0, Q42502, Q3YJT2 | | antibody format | | rabbit | polyclonal | | serum | lyophilized | | | quantity | | 200 µl | for reconstitution add 200 µl, of sterile water. | | | storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | tested applications | | western blot (WB), immunoprecipitation (IP) | | related products | | collection of antibodies to Solanum tuberosum | | additional information | | | |
application information
|
| recommended dilution | | 1 : 2000 with standard ECL (WB) |
| expected | apparent MW | | 40-42 kDa |
| confirmed reactivity | | Solanum tuberosum
|
| predicted reactivity | | Solanum tuberosum |
| not reactive in | | no confirmed exceptions from predicted reactivity are currently known |
| additional information | | |
| selected references | | to be added when available, antibody released in October 2012. |
application example
75 µg of total protein from Solanum tuberosum v. Nicola extracted with 20 mM TRIS PH-8.5, 10 mM thiourea,10mM CaCl2, 5mM DTT, 1 mM PMSF, 1%PVPP were separated on 4-20 % SDS-PAGE and blotted 1.5h to PVDF. Blots were blocked with 5% milk powder in T-TBS (2.5gr of powder in 50 ml T-TBS) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for overnight at 4°C with agitation. The antibody solution was decanted and the blot was washed 6 times for 5 min. in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:10 000 in blocking solution for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 30 seconds.
Courtesy of Dr. Paula Teper-Bamnolker and Dr. Dani Eshel, The Volcani Center, Israel
bio-equip.com
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