| product information
| | background | | Plant NADH dependent isocitrate dehydrogenase enzyme is located in mitochondrial matrix. This enzyme is classified as an oxidoreductase and its function is to catalyze a reaction in the citric acid cycle, specifically the sequential dehydrogenation and decarboxylation of isocitrate to form a-ketoglutarate. It removes hydrogens from its substrate, isocitrate. In addition to this process, it functions as a decarboxylase, removing a CO2 from the six-carbon substrate to form a five-carbon product mentioned above as a-ketoglutarate. There are two forms of this enzyme NADP+ and NAD+ dependent. | | immunogen | | KLH-conjugated peptide 1 and peptide 2 conserved in all higher plants mitochondrial, NAD dependent isocitrate dehydrogenase subunits including Arabidopsis thaliana IDH-I Q8LFC0, At4g35260 and IDH-II P93032, At2g17130 | | antibody format | | rabbit | polyclonal | | affinity purified serum, in PBS pH 7.4 | lyophilized | | | quantity | | 180 µg | for reconstitution add 180 µl of sterile water. | | | storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | tested applications | | western blot (WB) | | related products | | AS04 054 | anti-AOX1/2 rabbit antibody, marker of inner mitochondrial membrane AS04 053A | anti-COXII rabbit antibody marker of inner mitochondrial membrane AS07 212 | anti-VDAC1 rabbit antibody marker of outer mitochondrial membrane | | additional information | | Peptide used to elicit this antibody is not conserved in NADPH dependent anzymes, partially conserved across eukaryotic Idh subunits. Some conservation across bacterial which contain the NAD-dependent form of Idh (as opposed to the NADP-dependent form). | |
application information
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| recommended dilution | | 1 : 5 000 with standard ECL (WB) |
| expected | apparent MW | | 39 | 45 kDa (Arabidopsis thaliana) |
| confirmed reactivity | | Arabidopsis thaliana, Capsicum annuum, Lycopersicum chilense, Lycopersicum esculentum, Pisum sativum, Solanum sogarandium, Solanum tuberosum, Zea mays
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| predicted reactivity | | dicots including Brassica napus, Vitis vinifera, monocots including Oryza sativa, Zea mays |
| not reactive in | | Chlamydomonas reinhardtii |
| additional information | | cellular [compartment marker] of mitochondrial matrix |
| selected references | | Szabala et al. (2014). Accumulation of acidic SK3 dehydrins in phloem cells of cold- and drought-stressed plants of the Solanaceae. Planta, Jan 7. Lee et al. (2012). Mitochondrial Targeting of the Arabidopsis F1-ATPase γ-Subunit via Multiple Compensatory and Synergistic Presequence Motifs. Plant Cell, dec. 18. |
application example
20 µg of total protein from (1) Arabidopsis thaliana leaf extract, (2) Arabidopsis thalianafraction enriched with mitochondria, (3) Arabidopsis thaliana pure mitochondria, (4) Pisum sativum pure mitochondria, (5) Solanum tuberosum pure mitochondria were separated on 4-12% SDS-PAGE and blotted to nitrocellulose. Blots were blocked immediately following transfer in 5% milk powder in TBS. Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation. Blots were developed using E
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北京啟維益成科技有限公司（Beijing QiWei YiCheng Tech.,Ltd.(Qwbio)）成立于2005年12月，由從事生物領域多年銷售經(jīng)驗人士創(chuàng)立。目前我公司已經(jīng)與幾十家著名的試劑供應商建立了長期穩(wěn)定的合作關系，已成為眾多國際知名品牌的中國總代理或一級代理商；并且為滿足國內(nèi)客戶的最新需求，我們也與時俱進地不斷與世界上更多的知名品牌洽談合作代理事宜；企業(yè)的主要目標是把國外有特點的生物前沿產(chǎn)品引進中國，讓國內(nèi)科學研究機構和制藥企業(yè)盡早使用上最新型的科研產(chǎn)品。
我們代理的產(chǎn)品覆蓋細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發(fā)生產(chǎn)等領域。
細胞生物學包含細胞培養(yǎng)，細胞遷移，細胞信號轉導等。
植物學包含植物抗體，植物抗生素，植物組織培養(yǎng)試劑等。
分子生物學包含表觀遺傳試劑盒，核酸提取，轉染試劑等。
免疫學包含抗體，各類檢測試劑盒等。
生物化學包含各類生化試劑，化學小分子，常規(guī)試劑等。
蛋白組學包含不同種屬的重組蛋白，天然提取蛋白，細胞因子等。
生物制藥與診斷試劑研發(fā)方面的試劑包含各類蛋白抑制劑，酶類等。
在公司全體員工的共同努力和國外生產(chǎn)商的支持下，在業(yè)務上實現(xiàn)了迅猛發(fā)展，相繼在上海、武漢、廣州、長春、石家莊、哈爾濱等重點城市建立了分支機構或授權服務商，并以專業(yè)和優(yōu)質(zhì)的服務成為多家世界500強企業(yè)的中國區(qū)產(chǎn)品供應商，以及中國科學院科研采購平臺的中標供貨商。2008年以后業(yè)務的迅猛發(fā)展促進了啟維益成內(nèi)部組織架構的不斷優(yōu)化，我們建立了自己的網(wǎng)絡銷售平臺，業(yè)務代表銷售平臺，針對科研客戶與企業(yè)客戶的不同需求，綜合網(wǎng)絡平臺和業(yè)務代表平臺的優(yōu)點，啟維益成創(chuàng)建了科研用戶與工業(yè)客戶兩支獨立的營銷團隊，分別配備不同的專業(yè)技術支持和客服人員，通過網(wǎng)絡和業(yè)務代表，力求為不同類型的客戶提供前沿專業(yè)的解決方案。
公司客戶遍布中國生命科學基礎研究、開發(fā)應用、制藥、疾病診斷與控制、商品檢驗檢疫、生物技術公司、食品安全以及農(nóng)林畜牧業(yè)等諸多領域。
我們追求目的是與研究機構及合作伙伴之間誠信而專業(yè)的對話，力求建立長久合作。我們相信我們精心提供的高效、優(yōu)質(zhì)的產(chǎn)品和技術服務，會使您實驗設計更有效，實驗操作更簡便，研究結果更可靠。
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