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| application information | ||
| recommended dilution | 1 : 1000, detected with standard ECL (WB) | |
| expected | apparent MW | 20 | 20 kDa for Arabidopsis thaliana | |
| confirmed reactivity | Arabidopsis thaliana | |
| predicted reactivity | Betula luminifera, Brassica napa, Hordeum vulgare, Oryza sativa, Populus tomentosa, Solanum tuberosum, Zea mays | |
| not reactive in | no confirmed exceptions from predicted reactivity known in the moment | |
| additional information | note that detection for this product is limited by target threshold level | |
| selected references | to be added when available | |
application example 35 µg of total leaf protein extracted with PEB (AS08 300) from wt Arabidopsis thaliana (1) and Arabidopsis thaliana transformed with 35S::FT (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 80 min (30V) to PVDF. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-FT/TFT (AS06 198, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (300s, high sensitivity). Please note that this antibody will not detect FT at 35 µg protein loading in the wt leaf material tested. |
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