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GA3 | gibberellic acid
英文名稱:GA3 | gibberellic acid總訪問:464
國產(chǎn)/進口:進口半年訪問:1
產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
規(guī)       格:AS06194 最后更新:2024-12-5
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product information
background

Gibberellic Acid (GA) is a very potent plant hormone, which regulates the growth rate of plants promoting cell elongation. Gibberellic acid stimulates the cells of germinating seeds to produce mRNA molecules that code for hydrolytic enzymes. It was first discovered in Japan, in 1935 as a result of the study of a condition common in rice plants called "foolish seedling" disease, which caused the plants to grow much taller than normal.

immunogen

BSA-conjugated gibberellic acid (gibberellin A3)

antibody format

rat

polyclonal

total IgG in PBS, Phosphate 10mmol/l ; NaCl 150mmol/l at pH 7.35

lyophilized

quantity

100 µl,

for reconstitution add 50 µl, of sterile water and 50 µl of glycerol

storage

store lyophilized/reconstituted at -20°C; this aliquote can be freezed and thawed for up to five times and showed stability for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

immunolocalization (IL)

related products

AS06 193 | anti-indole acetic acid rabbit antibody

AS06 195 | anti-abscisic acid rabbit antibody

AS010 933 | GA3-BSA | gibberellic acid BSA conjugate

collection of antibodies to plant hormones

secondary antibodies to rat IgG

additional information

Total IgG concentration is 20 mg/ml. Antibody has low affinity to free GA and other GA forms: 1,4,5,7,9,53 as tested by competitive ELISA.

Using a conjugated Gibberellic acid 3-Ethyl-Chloroformiate-(PC), antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compound Cross-reactivity ratio (a) Gibberellic acid 3-ECF-BSA (GA3-BSA) 1 Gibberellic acid 1-ECF-BSA (GA1-BSA) 1/> 50 000 Gibberellic acid 4-ECF-BSA (GA4-BSA) 1/> 50 000 Gibberellic acid 5-ECF-BSA (GA5-BSA) 1/> 50 000 Gibberellic acid 7-ECF-BSA (GA7-BSA) 1/> 50 000 Gibberellic acid 9-ECF-BSA (GA9-BSA) 1/> 50 000 Gibberellic acid 53-ECF-BSA (GA53-BSA) 1/> 50 000 (a) : Gibberellic acid 3-ECF-BSA concentration/Other conjugated acids concentration at half displacement ; ECF = Ethyl-Chloroformiate, BSA : Bovine Serum Albumin.

GA3 antibody specificity testing by ELISA

application information
recommended dilution

1: 100 (IL)

expected | apparent MW

n.a.

confirmed reactivity

Euphorbia pulcherrima

predicted reactivity

dicots and monocots

not reactive in

no confirmed exceptions from predicted reactivity known in the moment

additional information

for detailed immunolocalization protocol check "more information"

selected references

Löfkeet al. (2013). Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters during root gravitropism. PNAS January 9.


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