| product information
| | background | | Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development. | | immunogen | | BSA-conjugated synthetic indole 3 acetic acid | | antibody format | | rabbit | polyclonal | | total IgG in PBS, Phosphate 10mmol/l ; NaCl 150mmol/l at pH 7.35 | lyophilized | | | quantity | | 100 µl, | for reconstitution add 50 µl, of sterile water and 50 µl of glycerol | | | storage | | store lyophilized/reconstituted at -20°C; this aliquote can be freezed and thawed for up to five times and showed stability for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | tested applications | | ELISA (ELISA), immunolocalization (IL) | | related products | | AS09 445 | anti-indole- 3- acetic acid (C1') for immunolocalization AS09 420 | anti-indole 3 acetic acid (C1') (for ELISA) AS09 421 | anti-indole 3 acetic acid (N1) collection of antibodies to plant hormones | | additional information | | to be added when available | |
application information
|
| recommended dilution | | 1: 5000 - 1: 10 000 (ELISA), 1:100 - 1:600 (IL) |
| expected | apparent MW | | n.a. |
| confirmed reactivity | | Arabidoppsis thaliana, Euphorbia pulcherrima, Medicago sativa (nodules)
|
| predicted reactivity | | dicots and monocots |
| not reactive in | | no confirmed exceptions from predicted reactivity known in the moment |
| additional information | | for detailed immunolocalization protocol check here. quantification of IAA in ELISAusing this antibody is described inBianco and Defez(2009). |
| selected references | | Bianco and Defez (2009). Medicago truncatula improves salt tolerance when nodulated by an indole-3-acetic acid-overproducing Sinorhizobium meliloti strain.J Exp. Bot. 60, No. 11: 3097-3107.
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application example
4 days old Arabidopsis thaliana seedlings were treated with 1µM NPA for 24 hours. Seedlings were fixed for 20 min in 4% EDAC in MTSB, and next 30 minutes in 4 % EDAC+ 2% Formaldehyde. Anti-auxin anti-rabbit primary antibody (Agrisera, catalog number AS06 193) diluted 1: 600 plus Goat anti-rabbit IgG (H&L), DyLight® 549 Conjugate (AS09 633) as secondary antibody diluted in 1: 3000 (shown in red color) were used.
Courtesy Dr. Taras Pasternak, Freiburg University, Germany.
bio-equip.com
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