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SPS | sucrose phosphate synthase, maize
英文名稱:SPS | sucrose phosphate synthase, maize總訪問:248
國產(chǎn)/進口:進口半年訪問:2
產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
規(guī)       格:AS06185 最后更新:2024-12-5
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product information
background

SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.

immunogen

Synthetic peptide derived from Zea mays SPS protein sequence (P31927).

antibody format

rabbit

polyclonal

total IgG in PBS pH 7.4

lyophilized

quantity

100 µl

- for reconstitution add 100 µl of sterile water

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products

AS03 035A SPS | sucrose phosphate synthase, global

for other antibodies to various maize proteins, please inquire.

additional information

total IgG concentration is 3 µg/µl

application information
recommended dilution

1 : 2 000 with standard ECL (WB)

expected | apparent MW

120 | ~130 for Zea mays

confirmed reactivity

Zea mays

predicted reactivity

monocots:Saccharum officinarum, Triticum aestivum and Oryza sativa

not reactive in

Hordeum vulgare

additional information

to be added when available

selected references

Sparks et al. (2001) A Appl Biol 138: 33-45.Potential for manipulating carbon metabolism in wheat


application example

10 µg of total leaf protein from (1) A.thaliana, (3) Zea mays and (4) Hordeum vulgare extracted with PEB (AS08 300) as well as 10 µg cytosolic protein from (2) A.thaliana were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SPS (AS06 0185, 1:2000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (90s, high sensitivity).

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