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當(dāng)前位置 > 產(chǎn)品目錄 > 試劑 > 植物試劑 > cAPX | cytosolic ascorbate peroxidase
cAPX | cytosolic ascorbate peroxidase
英文名稱：cAPX | cytosolic ascorbate peroxidase總訪問：390
國產(chǎn)/進(jìn)口：進(jìn)口半年訪問：1
產(chǎn)地/品牌：agrisera產(chǎn)品類別：植物試劑
規(guī)       格：AS06180 最后更新：2024-12-5
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product information
background

Ascorbate peroxidase (APX) is the enzyme catalyzing the ascorbate-dependent reduction of hydrogen peroxide. Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in fruits and photosynthetic tissues. AA is found in every compartment of the plant cell including the apoplast.
immunogen

KLH-conjugated peptide derived from N-terminal of Zea mays cytosolic APX Q41772
antibody format

rabbit

polyclonal

total IgG in PBS pH 7.4

lyophilized
quantity

200 µl

for reconstitution add 200 µl of sterile water.
storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications

western blot (WB), immunoprecipitation (IP), immunolocalization (IL)
related products

AS06 184 | anti-AO | apoplastic ascorbate oxidase

AS09 384 | anti-AO | ascorbate oxidase

AS09 383 | anti-AO | ascorbate oxidase, biotinylated antibody

AS08 368 | anti-APX | ascorbate peroxidase
additional information

total IgG concentration is 3 µg/µl
application information
recommended dilution

1:10 000 with standard ECL (WB), 2 µg (IP), 1: 500 (IL)
expected | apparent MW

28 kDa
confirmed reactivity

Arabidopsis thaliana (immunohistochemistry only) D. salina, A. toxicaria, Horderum vulgare, Pisum sativum, Silene vulgaris, S. lycopersicum, , Solanum tuberosum, P. silvestris, Zea mays
predicted reactivity

dicots including: Brassica. juncea, Fragaria ananassa, Gossypium hirsutum, Nicotiana tabacum,, Solanum tuberosum, Vitis vinifera, monocots including: Oryza sativa, trees: Pinus pinaster
not reactive in

no confirmed exceptions from predicted reactivity known in the moment
additional information
selected references Tsaniklidis et al. (2013). L-Ascorbic acid metabolism in parthenocarpic and seeded cherry tomatoes. Plant Growth Regul,DOI 10.1007/s10725-013-9845-0. (Solanum lycopersicum, immunolocalization)

Correa-Aragunde et al. (2013). Auxin induces redox regulation of ascorbate peroxidase 1 activity by S-nitrosylation/denitrosylation balance resulting in changes of root growth pattern in Arabidopsis. J Exp Bot, Aug;64(11):3339-49.

Wu et al. (2013). Comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus. J. Proteomics, June 14th.

Sobrino-Plata et al. (2013). Specific stress responses to cadmium, arsenic and mercury appear in the
2 metallophyte Silene vulgaris when grown hydroponically. RSC Advances, Jan 24.

Bai et al. (2011). Nitric Oxide Enhances Desiccation Tolerance of Recalcitrant Antiaris toxicaria Seeds via Protein S-Nitrosylation and Carbonylation. PLoSONE.

application example

(A) , (B)- control antibody, anti-PIN1, (C,D) immunolocalization using anti-cAPX antibodies.

Courtesy of Dr. Taras Pasternak


immunolocalization using anti-cAPX antibodies
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