综合图区亚洲网友自拍|亚洲黄色网络|成人无码网WWW在线观看,日本高清视频色视频kk266,激情综合五月天,欧美一区日韩一区中文字幕页

English | 中文版 | 手機(jī)版 企業(yè)登錄 | 個人登錄 | 郵件訂閱
當(dāng)前位置 > 產(chǎn)品目錄 > 試劑 > 植物試劑 > Ramy 3D | Alpha-amylase isozyme 3D
Ramy 3D | Alpha-amylase isozyme 3D
英文名稱:Ramy 3D | Alpha-amylase isozyme 3D總訪問:229
國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:2
產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
規(guī)       格:AS10716 最后更新:2024-12-5
貨       號:
CAS   號:
參考報價:詢價
立即詢價 電話咨詢
[發(fā)表評論] [本類其他產(chǎn)品] [本類其他供應(yīng)商] [收藏]
銷售商: 北京啟維益成科技有限公司 查看該公司所有產(chǎn)品 >>
  • 產(chǎn)品介紹
  • 公司簡介
product information
background α-Amylases are hydrolytic enzymes responsible for the mobilization of the starch into metabolizable sugars. This process provides the energy for the growth of roots and shoots and is crucial during germination of cereal seeds.

These enzymes are coded by a multigene family and even thought other amylolytic enzyme participate in the process of starch breakdown, the contribution of α-amylase is the prerequisite for the initiation of this process. Ramy3D is one of the alpha amylases genes in the rice multigene family (Huang et al. Nucleic Acid Research 1990).

Synonymes:1,4-alpha-D-glucan glucanohydrolase

immunogen

KLH-conjugated synthetic peptide derived from known Oryza sativa P27933

antibody format

rabbit

polyclonal

affinity purified serum

lyophilized

quantity

100 µg

for reconstitution add 100 µl, of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products AS10 712 | AMY1A | Alpha-amylase
additional information

to be added when available

application information
recommended dilution

1 : 5000 with standard ECL (WB)

expected | apparent MW

50 kDa

confirmed reactivity

Oryza sativa

predicted reactivity

monocots

not reactive in

no confirmed exceptions from predicted reactivity known in the moment

additional information to be added when available
selected references

to be added when available, antibody released in December 2010.


application example
western blot detection  of Ramy3D in rice

25 µg of total protein from Oryza sativa seedlings (from 1 day to 5 days of imbibition on filter paper at 28°C) extracted with an SDS Extraction Buffer (60mM Tris-HCl pH 8.0, 2% SDS, 1,5% Sucrose) were separated on XT CRITERION 10%Bis-Tris (BioRad) SDS-PAGE and blotted 1h to PVDF. Blot was blocked immediately in milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a diluition of 1: 5000 in milk in TBS-T for 3h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG HRP conjugated, AS09 602) diluited 1:20 000 in milk in TBS-T for 50 min at RT and then washed as above and developed for 3 min with standard ECL. Images of the blot were obtained using BioSpectrum AC Imaging System (UVP). Exposure time was 30 min. The arrow indicates RAmy3D (around 50KDa).

Courtesy Valeria Banti and prof. Pierodomenico Perata, PlantLab, Scuola Superiore Sant'Anna

western blot detection of RAmy3D in rice

25 µg of total protein from Oryza sativa embryos and aleurones (espectively E and A in the figure) were treated with Glucose 100mM and GA3 for 24 hr and 48 hr. Then protein were extracted with an SDS Extraction Buffer (60mM Tris-HCl pH 8.0, 2% SDS, 1,5% Sucrose) and were separated on XT CRITERION 10%Bis-Tris (BioRad) SDS-PAGE and blotted 1h to PVDF. Blot was blocked immediately in milk in TBS-T for 1h at room temperature (RT) with

bio-equip.com
售后服務(wù)
相關(guān)視頻
資料下載
快速詢價登錄注冊在線詢價 (請留下您的聯(lián)系方式,以便供應(yīng)商聯(lián)系您)
* 姓  名:
* 地  區(qū):
* 單  位:
職  位:
* 手機(jī)/電話:
* E-mail:
請寄產(chǎn)品資料:
需要 不需要
請報價格:
需要報價 不需要報價
留  言:
驗證碼:
換一張
發(fā)表評論在線評論(0條)
手機(jī)版:Ramy 3D | Alpha-amylase isozyme 3D
搜索本類產(chǎn)品

您最近瀏覽過的產(chǎn)品
Copyright(C) 1998-2025 生物器材網(wǎng) 電話:021-64166852;13621656896 E-mail:info@bio-equip.com
立即詢價