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3-nitroY | nitrotyrosine (25 μl)
英文名稱：3-nitroY | nitrotyrosine (25 μl)總訪問：272
國(guó)產(chǎn)/進(jìn)口：進(jìn)口半年訪問：4
產(chǎn)地/品牌：agrisera產(chǎn)品類別：植物試劑
規(guī)       格：AS10706-25 最后更新：2024-12-5
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product information
background

Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species.
immunogen

BSA-conjugated 3-(4-Hydroxy-3-nitrophenyl
acetamido) propionic acid
antibody format

mouse

monoclonal

IgG2A

liquid
quantity

25 µl
storage

store at -20°C; avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications

western blot (WB), ELISA (ELISA), immunohistochemistry (IHC), immunoprecipitation (IP)
related products

AS10 706-100 | anti-nitrotyrosine mouse antibody, 100 µg
additional information

1 mg/ml of Protein G purified IgG2A in PBS pH 7.4, 0.09 % sodium azide, 50 % glycerol
application information
recommended dilution

needs to be determined for each application
expected | apparent MW

n.a.
confirmed reactivity

human, mouse, rat, dog
predicted reactivity

higher plants
not reactive in

no confirmed exceptions from predicted reactivity known in the moment
additional information

antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity
with non-nitrated tyrosine. Not species specific.

0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot using standard ECL
selected references

Antibody used in western blot and ELISA: Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387. Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.

Antibody used in immunohistochemistry: Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.

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