Description:

Rabbit polyclonal antibody

  Background:

The core of the photosystem I reaction center is formed by polypeptides PSI-A and PSI-B, the products of the homologous PsaA and PsaB genes. PsaB is a core protein of PSI complex.

  Synonyms:

PsaB, Photosystem I P700 chlorophyll a apoprotein A2, PSI-B

  Immunogen:

KLH-conjugated synthetic peptide of PsaB derived from Arabidopsis thaliana ATCG00340.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

82 / 63 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana, Oryza sativa, Zea mays , Glycine max, Hordeum vulgare subsp. Vulgare.

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Medicago truncatula, Chlamydomonas reinhardtii, Solanum tuberosum, Gossypium raimondii, Cucumis sativus, Sorghum bicolor, Setaria viridis, Panicum virgatum, Populus trichocarpa, Vitis vinifera, Synechocystis sp. PCC 6803, Nicotiana tabacum, Brassica napus, Solanum lycopersicum, Spinacia oleracea, Triticum aestivum, and 80-99% homologues with the sequence in Physcomitrella patens. 
For more species homologues information, please contact tech support at tech@phytoab.com.

1-3 is thylakiod membrane protein from Arabidopsis thaliana leaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.