| product information
| | background | | Pyruvate decarboxylase (PDC) is a homotetrameric enzyme (E.C.4.1.1.1) that catalyses the decarboxylation of pyruvic acid to acetaldehyde carbon dioxide in the cytoplasm. It is also called 2-oxo-acid carboxylase, and pyruvic decarboxylase. In anaerobic conditions, this enzyme is part of the fermentation process that occurs in yeast, especially the Saccharomyces genus, to produce ethanol by fermentation. Pyruvate decarboxylase starts this process by converting pyruvate into acetaldehyde and carbon dioxide. Pyruvate decarboxylase depends on cofactors thiamine pyrophosphate (TPP) and magnesium. This enzyme should not be mistaken for the unrelated enzyme pyruvate dehydrogenase, an oxidoreductase (EC 1.2.4.1), that catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA. | | immunogen | | KLH-conjugated peptide derived from available PDC sequences including Arabidopsis thaliana | | antibody format | | rabbit | polyclonal | | serum | lyophilized | | | quantity | | 100 µl | for reconstitution add 100 µl of sterile water | | | storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | tested applications | | western blot (WB) | | related products | | AS10 685 | ADH | alcohol dehydrogenase (hypoxia marker) | | additional information | | to be added when available | |
application information
|
| recommended dilution | | 1: 10 000 with standard ECL (WB) |
| expected | apparent MW | | 65 | 65 kDa (Arabidopsis thaliana) |
| confirmed reactivity | | Arabidopsis thaliana, Oryza sativa
|
| predicted reactivity | | dicots including: Brassica napus, Glycine max, Pisum sativum, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Vitis vinifera, monocots including: Hordeum vulgare, Zea mays |
| not reactive in | | no confirmed exceptions from predicted reactivity known in the moment |
| additional information | | to be added when available |
| selected references | | to be added when available, antibody released in September 2010 |
application example
20 µg of total protein from Arabidopsis thaliana seedlings (0-2-4-8-12-24 hours of anoxic treatment with aerobic control) of WT Col-0 extracted with an SDS Extraction Buffer (60mM Tris-HCl pH 8.0, 2% SDS, 1,5% Sucrose) were separated on XT CRITERION 10% Bis-Tris (BioRad) SDS-PAGE and blotted 1h to PVDF. Blot was blocked immediately in milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the anti-PCD antibodies at a diluition of 1: 10 000 in milk in TBS-T for 3h at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG HRP conjugated from Agrisera, AS09 602) diluited 1:20 000 in milk in TBS-T for 50 min at RT and then washed as above and developed for 2 min with standard ECL. Images of the blot were obtained using BioSpectrum AC Imaging System (UVP). Exposure time was 10 min.
20 µg of total protein from Orysa sativa coleoptiles (3-4-5-6 days of germination under aerobic and anoxic conditions) extracted with an SDS Extraction Buffer (60mM Tris-HCl pH 8.0, 2% SDS, 1,5% Sucrose) were
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