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                                                              TNFa | Tumor necrosis factor alpha, Biotin conjugated
                                                              英文名稱:TNFa | Tumor necrosis factor alpha, Biotin conjugated總訪問:466
                                                              國產(chǎn)/進口:進口半年訪問:10
                                                              產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                                              規(guī)       格:IMS06-102-325 最后更新:2024-12-5
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                                                              product information




                                                              background
                                                               

                                                              Tumor Necrosis Factor alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.




                                                              immunogen
                                                               

                                                              purified full length recombinant protein P01375




                                                              antibody format
                                                               



                                                              hen;


                                                              polyclonal;



                                                              affinity purified IgY in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1 % sodium azide, pH 7.2;


                                                              liquid







                                                              quantity
                                                               



                                                              200 µl








                                                              storage
                                                               

                                                              store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.




                                                              tested applications
                                                               

                                                              Western blot (WB), ELISA (ELISA)




                                                              related products
                                                               

                                                              IMS01-142-310 | Etanercept (Enbrel®), recombinant human tumor necrosis factor receptor




                                                              additional information
                                                               

                                                              the IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation; labelled with biotin.

                                                              Total IgY concentration for this antibody is 15 mg/ml






                                                              application information




                                                              recommended dilution
                                                               

                                                              1: 5000 with standard ECL (WB)




                                                              expected | apparent MW
                                                               

                                                              26 kDa




                                                              confirmed reactivity
                                                               

                                                              human




                                                              predicted reactivity
                                                               

                                                              cat, dog, horse, pig, primates




                                                              not reactive in
                                                               

                                                              no confirmed exceptions from predicted reactivity known in the moment




                                                              additional information
                                                               

                                                              the antibody, in dilution 1:5 000, will detect 10 ng of SDS denatured and reduced human TNFa on western blots




                                                              selected references
                                                               

                                                              to be added when available









                                                              ||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use LiveChat option in the left menu or contact us at [email protected]









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