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                                                              Rab11-GTP 小鼠單抗實驗使用及操作手冊

                                                              瀏覽次數(shù):875 發(fā)布日期:2023-3-16  來源:費斯德生物官網(wǎng)
                                                              Product Description
                                                              Pioneering GTPase and Oncogene Product Development since 2010
                                                              Rab11-GTP
                                                              Catalog Number: 26919
                                                              Gene Symbol: RAB11
                                                              Description: Anti-Rab11-GTP Mouse Monoclonal Antibody
                                                              Background: Small GTPases of the Rab-family are crucial elements of the machinery that controls
                                                              membrane traffic. Rab11 has been shown to control traffic through the recycling endosome.
                                                              Immunogen: Recombinant full length protein of active
                                                              Rab11
                                                              Tested Applications: IP, IHC
                                                              Recommended Dilutions:
                                                              IP: 1 g for 1~2 mg total cellular proteins.
                                                              Immunoprecipitation and Western blot:
                                                              IHC: 1:50~100 dilution
                                                              Concentration: 1 mg/ml
                                                              Host: Mouse
                                                              Clonality: Monoclonal
                                                              Isotype: IgG2a, IgG2b P
                                                              Purity: Purified from ascites
                                                              Format: Liquid
                                                              Preservative: No
                                                              2+2+
                                                              Constituents: PBS (without Mg and Ca ), pH 7.4, 150 mM NaCl, 50% glycerol
                                                              Species Reactivity: Anti- Rab11-GTP antibody
                                                              recognizes active Rab11 from vertebrates.
                                                              Storage Conditions: Store at -20°C. Avoid freeze /
                                                              thaw cycles
                                                              Rab11 Activation Assay.
                                                              Purified full-length Rab11 proteins were immunoprecipitated after treated with GDP (lane left) or GTPS (lane right). Immunoprecipitation
                                                              was done with the anti-Rab11-GTP monoclonal antibody (Cat. No. 26919). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. 21157).
                                                              FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC APPLICATIONS
                                                               
                                                              來源:武漢費斯德生物科技有限公司
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