Application of SMALP in Screening of Different Membrane Proteins
Description
The SMALP, among all synthetic nanodisc products has been developed for for the solubilization of membrane proteins from all kinds of cell membranes and subsequent isolation & purification of said membrane proteins. After dissolving SMALP 200 + buffer powder in dH2O, the product is ready-to-use. SMALP 200 has a yellowish color in solution. After successfull solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommed to use the Rho1D4-tag. Cube Biotech offers the matching products for this purpose.
Datasheets
Citations
Lab Results
SMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set. Furthermore if you like to compare proteins's performance between SMA and DIBMA in synthetic nanodiscs, we also offer our, that includes both our SMA and DIBMA products.
Figure 1: Western Blot of a purified membrane protein after stabilization through SMA. Reproduced with permission from Ms S. Nestorow, University of Birmingham.
The SMALP, among all synthetic nanodisc products has been developed for for the solubilization of membrane proteins from all kinds of cell membranes and subsequent isolation & purification of said membrane proteins. After dissolving SMALP 200 + buffer powder in dH2O, the product is ready-to-use. SMALP 200 has a yellowish color in solution. After successfull solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommed to use the Rho1D4-tag. Cube Biotech offers the matching products for this purpose.
Datasheets
| FEATURE | |
| State | Lyophilized Powder, to be solved with water |
| Polymer styrene-to-maleic anhydride ratio | 2:1 |
| Molecular weight | 6,500 Da |
| pH after solving | 7.5 |
| Solubility | 40 % |
| Divalent cationic tolerance | < 5 mM |
| Color | Yellowish |
| Odor | Odorless |
| Freeze-thaw stable? | Yes |
| Shipping Temperature | ambient temperature |
| Storage of lyophilized copolymer | -20°C for several years |
| Storage of dissolved copolymer | 2-8°C for several days |
| Other noteworthy feature | Most commonly used SMA |
| Structure |  |
Citations
| STABILIZED PROTEIN | YEAR | AUTHOR |
| Bacterial Membrane Proteins | 2022 | Noh I., Guo Z., Zhou J., Gao W., Fang R.H., Zhang L. |
| - | 2023 | Hernandez L.M.R. & Levental I. |
| Alpha-Toxin degrading proteins | 2023 | Sun L., Wang D., Noh R., Fang H., Gao W., Zhang L. |
| ATP-binding cassette (ABC) transporter | 2023 | Dimitrova V.A., Song S., Karagiaridi A., Marand A., Pinkett H.W. |
| Env-protein of HIV | 2023 | Zhou R., Zhang S., Nguyen H.T., Ding H., Gaffney A., Kappes J.C., Smith III. A.B.S, Sodroski J.G. |
Lab Results
SMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set. Furthermore if you like to compare proteins's performance between SMA and DIBMA in synthetic nanodiscs, we also offer our, that includes both our SMA and DIBMA products.
標簽：
蛋白純化;膜蛋白;SMALP
- IPHASE亮相第十四屆藥理學(xué)會藥物和化學(xué)異物代謝會議
- IPHASE亮相第十四屆藥物和化學(xué)異物代謝學(xué)術(shù)會議
- IPHASE助力區(qū)域創(chuàng)新發(fā)展活動暨產(chǎn)學(xué)研聯(lián)動創(chuàng)新交流會
- IPHASE課堂第三期：轉(zhuǎn)運體研究原理及設(shè)計
- 北京華創(chuàng)恒達科技進口一批QC-Beads質(zhì)控珠到貨
- 國內(nèi)首家：IPHASE ABC家族BCRP轉(zhuǎn)運體膜囊泡研發(fā)成功
- 本生生物推出：Elisa試劑盒“滿五送一”活動！
- IPHASE亮相中國毒理學(xué)會第十一次全國毒理學(xué)大會
Copyright(C) 1998-2025 生物器材網(wǎng) 電話：021-64166852;13621656896 E-mail：[email protected]