中文名稱細胞角蛋白7單克隆抗體

別    名Cytokeratin 7; CK 7; K7; Keratin 7; Keratin,type II cytoskeletal 7; KRT7; SCL; K2C7_HUMAN.  

研究領(lǐng)域腫瘤  信號轉(zhuǎn)導(dǎo)  

抗體來源Mouse

克隆類型Monoclonal

克 隆 號10E3

交叉反應(yīng)Human, Mouse,  (predicted: Rat, )

產(chǎn)品應(yīng)用WB=1:500-2000 IHC-P=1:100-500 （石蠟切片需做抗原修復(fù)）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量54kDa

細胞定位細胞膜 

性    狀Liquid

濃    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human CK7: 

亞    型IgG

純化方法affinity purified by Protein G

儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

產(chǎn)品介紹Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

 

Function:

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

 

Subunit:

Heterotetramer of two type I and two type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7.

 

Subcellular Location:

Cytoplasm.

 

Tissue Specificity:

Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.

 

Post-translational modifications:

Arg-20 is dimethylated, probably to asymmetric dimethylarginine.

 

Similarity:

Belongs to the intermediate filament family.

 

SWISS:

P08729

 

Gene ID:

3855

 

Database links:

Entrez Gene: 3855 Human

 

Omim: 148059 Human

 

SwissProt: P08729 Human

 

Unigene: 411501 Human

 

Unigene: 670221 Human 

 

 

 

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

結(jié)構(gòu)蛋白（Structural Proteins）

CK-7是一種 54KDa 的中間絲蛋白，存在于大多數(shù)正常組織的腺上皮和移行上皮細胞中。 該抗體與多種良/惡性上皮性腫瘤反應(yīng)。腺癌中的卵巢、乳腺、肺的腺癌呈陽性反應(yīng)，而胃腸道的腺癌陰性。移行細胞腫瘤、前列腺癌也呈陽性反應(yīng)。通常認為 CK7是腺癌和移行上皮細胞癌的比較特異性的標志

產(chǎn)品圖片

Sample: Hela（human）Cell Lysate at 40 ug MDA-MB-231（human） Cell Lysate at 40 ug Primary: Anti-CK7 (bsm-33060M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD Sample:A549(Human) Cell Lysate at 40 ug Primary: Anti-CK7 (bsm-33060M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD Paraformaldehyde-fixed, paraffin embedded (human pancreatic carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK7) Monoclonal Antibody, Unconjugated (bsm-33060M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK7) Monoclonal Antibody, Unconjugated (bsm-33060M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human esophagus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK7) Monoclonal Antibody, Unconjugated (bsm-33060M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK7) Polyclonal Antibody, Unconjugated (bsm-33060M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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