別    名 FOX3; NeuN; neuronal nuclei antigen; neuronal nuclei; Vertebrate neuron-specific nuclear protein; Neuna60; Neuronal nuclear antigen A60; RNA binding protein fox-1 homolog 3 isoform 1; RNA binding protein, fox 1 homolog (C. elegans) 3; Rbfox3; D11Bwg0517e; Fox-3; Fox3; Hrnbp3; RP23-159O6.5; novel protein; Fox-1 homolog C; fox1 homolog C; FLJ56884; FLJ58356; hexaribonucleotide binding protein 3 RFOX3_HUMAN.   

研究領域 細胞生物  神經生物學  轉運蛋白  表觀遺傳學   

抗體來源 Rabbit 

克隆類型 Monoclonal 

克 隆 號 12C3 

交叉反應 Mouse, Rat,  (predicted: Human, ) 

產品應用 WB=1:1000-2000 

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user. 

分 子 量 34kDa 

細胞定位 細胞核 細胞漿  

性    狀 Liquid 

濃    度 1mg/ml 

免 疫 原 KLH conjugated synthetic peptide derived from human NeuN:  

亞    型 IgG 

純化方法 affinity purified by Protein A 

儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 

保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. 

PubMed PubMed 

產品介紹 Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei) is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. NeuN is a neuron-specific, DNA-binding nuclear protein in vertebrates. In mice, NeuN is observed in most neuronal cell types throughout the nervous system, including cerebellum, cerebral cortex, hippocampus, thalamus and spinal cord, as well as the dorsal root ganglia, sympathetic chain ganglia and enteric ganglia of the peripheral nervous system. NeuN immunoreactivity is first observed in neurons when they become post-mitotic and are initiating cellular and morphological differentiation. No staining is observed in proliferative zones. NeuN has been used as an immunohistochemical marker for excitotoxic lesions of the brain as well as in the diagnosis of a wide range of human tissue specimens from the central and peripheral nervous systems.

 

Function:

RNA-binding protein t

產品圖片

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Cerebellum (Mouse) Lysate at 40 ug Lane 3: Cerebrum (Rat) Lysate at 40 ug Lane 4: Cerebellum (Rat) Lysate at 40 ug Primary: Anti-NeuN (bsm-52268R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46/50 kD Observed band size: 46/50 kD

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