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兔抗FtsZ
英文名稱:FtsZ | procaryotic cell division GTP總訪問:438
國產(chǎn)/進口:進口半年訪問:1
產(chǎn)地/品牌:agrisera產(chǎn)品類別:其他生物試劑
規(guī)       格:AS10715 最后更新:2024-12-5
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product information

background  

FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules. Synonymes: sifB, SulB.

immunogen  

KLH-conjugated synthetic peptide derived from known bacterial sequences of FtsZ including E.coli P0A9A6

antibody format  








rabbit


polyclonal


serum


lyophilized

quantity  





200 µl


for reconstitution add 200 µl, of sterile water.

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB)

related products  
additional information  

to be added when available


































application information

recommended dilution  

1 : 1000 with standard ECL (WB)

expected | apparent MW  

40 | 42 kDa

confirmed reactivity  

E coli DH5a, Shigella flexneri

predicted reactivity  

Candidatus sp., Citrobacter sp. 30_2, Dickeya sp., Enterobacter sp., Klebsiella pneumoniae subsp. pneumoniae MGH, Salmonella sp., Shigella sonnei Ss046, Vibrio sp., Yersinia pestis D182038

not reactive in  

B. subtilis, Listera sp. cyanobacteria

additional information  

to be added when available

selected references  

to be added when available, antibody release in October 2011.


application example

western blot detection of bacterial FtsZ protein using anti-FtsZ antibody

 5 µg of total protein from Synechocystis sp. (1), E.coli DH5a (2), E. coli (3),  extracted with Agrisera PEB extraction buffer were separated on  4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked with Advance blocking reagent  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 120 seconds.

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