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                                                              CP43’ | IsiA homolog of plant CP43 protein standard
                                                              英文名稱:CP43‘ | IsiA homolog of plant CP43 protein standard總訪問:228
                                                              國(guó)產(chǎn)/進(jìn)口:進(jìn)口半年訪問:1
                                                              產(chǎn)地/品牌:agrisera產(chǎn)品類別:抗體
                                                              規(guī)       格:AS10111S 最后更新:2024-12-5
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                                                              product information
                                                              background

                                                              This product is a recombinant protein standard, source: Synechocystis strain PCC 6803.

                                                              immunogen
                                                              antibody format
                                                              does not apply
                                                              quantity

                                                              250 μl

                                                              lyophilized, for reconstitution add 225 μl of milliQ water

                                                              storage

                                                              store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                                              tested applications

                                                              western blot (WB)

                                                              related products

                                                              AS06 111 | anti-CP43' | IsiA homolog of plant CP43 antibodies

                                                              Collection of global antibodies

                                                              Collection of antibodies to photosynthetic proteins

                                                              additional information

                                                              The IsiA protein standard can be used in combination with anti-IsiA antibodies to quantitate IsiA from a range of cyanobacteria. Global antibodies are raised against highly conserved amino acid sequences in theIsiA protein.

                                                              Quantitative western blot: detailed method description, video tutorial

                                                              application information
                                                              recommended dilution

                                                              Standard curve: 3 loads are recommended (2.5 and 10 μl).
                                                              For most applications a sample load of 0.2μg of chlorophyll will give a IsiA signal in this range.

                                                              Positive control:a 2μl load per well is optimal for most chemiluminescent detection systems.

                                                              This standard is stabilized and ready and does not require heating before loading on the gel.

                                                              expected | apparent MW

                                                              27 kDa (slightly larger than native protein due to His-tag)

                                                              confirmed reactivity
                                                              predicted reactivity
                                                              not reactive in
                                                              additional information

                                                              Concentration: after adding 225 μl of milliQ water final concentration of the standard is 0.15 pmoles/μl

                                                              Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.

                                                              This standard is ready-to-load and does not require any additions or heating.

                                                              selected references Fraser et al. (2013). Photophysiological and Photosynthetic Complex Changes during Iron Starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942.PLoS ONE 8(3): e59861. doi:10.1371/journal.pone.0059861

                                                              Ryan-Keogh et al. (2012). Iron deficiency in cyanobacteria causes monomerization of photosystem I trimers and reduces the capacity for state transitions and the effective absorption cross section of photosystem I in vivo. J. of Phycology, 1:145-154.

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