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CP43’ | IsiA homolog of plant CP43 protein standard
英文名稱：CP43‘ | IsiA homolog of plant CP43 protein standard總訪問：228
國(guó)產(chǎn)/進(jìn)口：進(jìn)口半年訪問：1
產(chǎn)地/品牌：agrisera產(chǎn)品類別：抗體
規(guī)       格：AS10111S 最后更新：2024-12-5
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product information
background

This product is a recombinant protein standard, source: Synechocystis strain PCC 6803.
immunogen
antibody format
does not apply
quantity

250 μl

lyophilized, for reconstitution add 225 μl of milliQ water
storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications

western blot (WB)
related products

AS06 111 | anti-CP43' | IsiA homolog of plant CP43 antibodies

Collection of global antibodies

Collection of antibodies to photosynthetic proteins

additional information

The IsiA protein standard can be used in combination with anti-IsiA antibodies to quantitate IsiA from a range of cyanobacteria. Global antibodies are raised against highly conserved amino acid sequences in theIsiA protein.

Quantitative western blot: detailed method description, video tutorial
application information
recommended dilution

Standard curve: 3 loads are recommended (2.5 and 10 μl).
For most applications a sample load of 0.2μg of chlorophyll will give a IsiA signal in this range.

Positive control:a 2μl load per well is optimal for most chemiluminescent detection systems.

This standard is stabilized and ready and does not require heating before loading on the gel.
expected | apparent MW

27 kDa (slightly larger than native protein due to His-tag)
confirmed reactivity
predicted reactivity
not reactive in
additional information

Concentration: after adding 225 μl of milliQ water final concentration of the standard is 0.15 pmoles/μl

Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.

This standard is ready-to-load and does not require any additions or heating.
selected references Fraser et al. (2013). Photophysiological and Photosynthetic Complex Changes during Iron Starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942.PLoS ONE 8(3): e59861. doi:10.1371/journal.pone.0059861

Ryan-Keogh et al. (2012). Iron deficiency in cyanobacteria causes monomerization of photosystem I trimers and reduces the capacity for state transitions and the effective absorption cross section of photosystem I in vivo. J. of Phycology, 1:145-154.

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