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| application information | ||
| recommended dilution | 1 : 75 000 with standard ECL (WB) | |
| expected | apparent MW | 15.9 kDa | |
| confirmed reactivity | human | |
| predicted reactivity | bovine, chimpanzee, dog, goat, guinea pig, mouse, pig, rabbit, rat, sheep, fission yeast | |
| not reactive in | no confirmed exceptions from predicted reactivity known in the moment | |
| additional information | Peptide used to elicit this antibody is not conserved in SOD2,3 and 4. | |
| selected references | antibody available in October 2009 | |
| application example 10 µl of control mouse brain and a human spinal ventral horn were extracted in 25 volumes of PBS, diluted 1+1 in a sample buffer have been loaded per well and separated on 15% SDS-PAGE and blotted for 1h to PVDF. Blots were blocked immediately following transfer in 5% Dry milk for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 75 000 (AS09 537) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 5 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, from DAKO PO448) diluted to 1:42 000 for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager. Exposure time was 30-600 seconds. |   |
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