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                                                              PEN2 | gamma-secretase subunit PEN-2
                                                              英文名稱:PEN2 | gamma-secretase subunit PEN-2總訪問:178
                                                              國產(chǎn)/進口:進口半年訪問:1
                                                              產(chǎn)地/品牌:agrisera產(chǎn)品類別:抗體
                                                              規(guī)       格:AS06154 最后更新:2024-12-5
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                                                              background

                                                              One characteristic feature of Alzheimer’s disease is the deposition of amyloid-beta peptide (Abeta) as amyloid plaques within specific regions of the human brain. Abeta is derived from the amyloid-beta peptide precursor protein (beta-APP) by the intramembranous cleavage activity of gamma-secretase. PEN-2 (Q9NZ42) is a critical component of PS1/gamma secretase (Farmery et al. 2003; Bergman et al. 2004).Alternative name: presenilin enhancer protein 2

                                                              immunogen

                                                              the antibody was raised against the N-terminal eleven residues of human PEN-2. PEN-2 is the smallest component of the multi-protein gamma-secretase complex. Chosen synthetic peptide has been conjugated to KLH

                                                              antibody format

                                                              rabbit;

                                                              polyclonal;

                                                              affinity purified IgG;

                                                              lyophilized

                                                              quantity

                                                              200 µg

                                                              - for reconstitution add83.3 µl of sterile water.

                                                              storage

                                                              store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                                              tested applications

                                                              Western blot (WB)

                                                              related products

                                                              Alzheimer | available antibodies for Alzheimer's disease

                                                              additional information

                                                              to be added when available

                                                              application information
                                                              recommended dilution

                                                              1:250 (WB)

                                                              expected | apparent MW

                                                              15 kDa

                                                              confirmed reactivity

                                                              human, mouse, hamster

                                                              predicted reactivity

                                                              bovine, rat, Xenopus

                                                              not reactive in

                                                              no confirmed exceptions from predicted reactivity known in the moment

                                                              additional information

                                                              approx. MW of the target protein is 15 kDa. Antibodies recognize also an unknown protein at around 40 kDa

                                                              selected references

                                                              to be added when available


                                                              Western blot experiment example


                                                              Rat brain homogenate (15 µg). Overnight incubation of PVDF membrane with Pen-2 antibody (5 µg/ml) in PBS containing 1% BSA. Horseradish peroxidase-conjugated anti-rabbit antibody (0.5 µg/ml, GE Biosciences) was used for detection.

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