Description:

Rabbit polyclonal antibody

  Background:

ATPase alpha subunit, which is a subunit of ATP synthase and part of the CF1 portion which catalyzes the conversion of ADP to ATP using the proton motive force. This complex is located in the thylakoid membrane of the chloroplast.

  Synonyms:

AtpA, ATP synthase F1 sector subunit alpha, F-ATPase subunit alpha.

  Immunogen:

KLH-conjugated synthetic peptide of AtpA derived from Arabidopsis thaliana ATCG00120.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

55 / 59 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Glycine max, Physcomitrium patens, Solanum lycopersicum, Vitis vinifera, Gossypium raimondii, Brassica napus, Cucumis sativus, Solanum tuberosum, Nicotiana tabacum, Populus trichocarpa, Vitis vinifera, Spinacia oleracea, and 80-99% homologues with the sequence in Zea mays, Medicago truncatula, Panicum virgatum, Oryza sativa, Synechococcus elongatus.
For more species homologues information, please contact tech support at tech@phytoab.com.

Thylakoid membrane protein from Arabidopsis thaliana containing 0.5 μg of chlorophyll.
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.