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application information | ||
recommended dilution | 1: : 80 000 (ELISA) | |
expected | apparent MW | n.a. | |
confirmed reactivity | GnRHa | |
predicted reactivity | GnRHa | |
not reactive in | no confirmed exceptions from predicted reactivity known in the moment | |
additional information | In ELISA immunoassay anti-GnRHa antibody and sample/standard are added simultaneously within GnRHa coated wells. After incubation, anti-GnRHa antibody bound to the coated GnRHa is detected with HRP-labelled secondary antibodies and color development achieved by addition of TMB reagent. | |
selected references | Guzmán et al. (2008). Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis. General & Comparative Endocrinol. 156: 285-297. |