This blocking peptide can be used as a control to neutralizeHalorhodopsin before immunolocalization or western blot. Furter details are provided below.

Halorhodopsin (HR) is a hyperpolarizing light-driven ion pump from the halophilic archaea bacterium Natronomonas pharaonis. HR uses the energy of yellow light (excitation maximum near 580 nm) to mediate primarily chloride but also bromide, iodide, and nitrate import into the cell against their electrochemical gradients.

does not apply

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Neutralization assay

AS12 1851Halorhodopsin

Estimation of the ratio between antibodies and a peptide : In neutralisation assay use0,73 µl ofHalorhodopsin peptide for 10 ml of reaction volume.
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