| product information
| | background | | HliD (ScpE) protein, high light inducible protein, is LHC-similar stress-induced protein from cyanobacteria. HliD is associated with damaged photosystem II and can serve as a temporary pigment reservoir while photosystem II components are being replaced.
| | immunogen | | synthetic peptide (amino acids 15 – 30) for HliD protein from Synechocystis sp. PCC 6803. NP_440269.1
| | antibody format | | | rabbit | polyclonal | | serum | liquid | | | quantity | | | | storage | | Short term 4°C. Long term -20°C. Repeated freezing and thawing is not recommended. Solution contains 0.01% sodium azide. | | tested applications | | western blot (WB) | | related products | | AS10 1615 | nti-HliD | High light inducible protein, larger pack size | | additional information | | Using nitrocellulose for western blotting is highly recommended to obtain a signal with this antibody. Pre-immune serum is available to this product upon request. | |
application information
|
| recommended dilution | | 1: 2000 with standard ECL (WB) |
| expected | apparent MW | | 5 kDa
|
| confirmed reactivity | | Synechocystis sp. PCC 6803 |
| predicted reactivity | | According to sequence analysis antibody may react with homologous Hli protein(-s) from Anabaena, Thermosynechococcus, Gloeobacter, Prochlorococcus, Synechococcus and Crocosphaera. |
| not reactive in | | no confirmed exceptions from predicted reactivity known in the moment |
| additional information | | to be added when available |
| selected references | | to be added when available |
application example
40 µg of membrane proteins from Synechocystis 6803 WT and DhliD strains was separated on 12-20 % SDS-PAGE and blotted 3h to PVDF. Blot was blocked with 0.2% Tween 20 in TBS for 1h at room temperature with agitation and than incubated in the primary antibody at a dilution of 1: 2 000 overnight at 10°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed twice for 5 min in TBS-T at room temperature with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 10 000 in for 1h at room temperature with agitation. Finally, the blot was washed as above, developed for 5 min with ECL (Plus Supersignal West Pico) according to the manufacturers instructions and exposed for 30 min in LAS 4000 imager.
WT and ΔScpE deletion mutants grown at 40 μE m-2s-1 and exposed to HL 2000 μE m-2s-1 for 30 min.
Courtesy of Dr. Josef Komenda, Academy of Science of Czech Republic
bio-equip.com
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